ANTIBACTERIAL ACTIVITY OF ADHATODA VASICA PDF

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Adhatoda vasica belonging to Acanthaceae family, commonly known as Adosa, Adhatoda vasica Flowers, Antibacterial activity, Antifungal activity, Diffusion. Abstract. The present study aimed to evaluate the in vitro antimicrobial activity of pharmacologically important Adhatoda vasica plant extracts. Antibacterial activity of leaf extract of Adhatoda vasica was studied against Bacillus subtilis, Antibacterial activity was determined by disc diffusion method.

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The plant is reported to show abortifacient [ 7 ], antimicrobial [ 89 ], and antitussive activities [ 10 ]. Open in a separate window. Minimum Inhibitory Concentration MIC Minimum inhibitory concentration studies of the compounds were performed according to the standard reference methods for bacteria [ 17 ] and filamentous fungi [ 18 ].

Then the residue was crystallised from chloroform to get Vasicine acetate as colorless crystals m. The structure was elucidated using spectroscopic methods: The cytotoxicity was determined according to the method of Balachandran et al.

The MIC for fungi was defined as the lowest extract concentration, showing no visible fungal growth after incubation time. Conclusion Vasicine acetate was obtained by acetylation of Vasicine recovered from A.

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The crude extract of A. Investigation on antifeedant and anthelmentic potential of Adhatoda vasica nees. Kulkarni [ 24 ] reported that A.

Conflict of Interests The authors declare that there is no conflict of interests regarding the publication of this paper. Please review our privacy policy. Sankar2 and K.

Accumulation of vasicine and vasicinone in tissue cultures of Adhatoda vasica and evaluation of the free radical-scavenging activities of the various crude adhatodq. The plant was identified by Dr. International Journal of Green Pharmacy. The mobile phase was filtered through a 0. Xdhatoda MIC for bacteria was determined as the lowest concentration of the compound inhibiting the visual growth of the test cultures on the agar plate.

Ignacimuthu123 C. The alkaloid Vasicine was isolated from ethanolic extract of the leaves of A. Nees Acanthaceaeknown commonly as Malabar nut tree, is a shrub growing throughout the Indian peninsula.

Greater antibacterial activity against Pseudomonas aeruginosa was only exhibited by Phyllanthus emblica, Pinus roxburghii, Debregeasia salicifolia and Lantana camara. UV-Visible spectra were taken in methanol on Thermo Fisher instrument.

The solutions were filtered through a membrane filter pore size adhhatoda. Separation was carried out at room temperature. Indian Journal of Medical Research. Essential oils of the leaves of A.

Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Minimum inhibitory concentration studies of the compounds were performed according to the standard reference methods for bacteria [ 17 ] and filamentous fungi [ 18 ].

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Mobile phase A consisted of water with 0.

Indian Journal of Natural Products and Resources. Vasicine acetate could be probed further in drug discovery programme.

The column was eluted with chloroform and chloroform: Structure of the compounds: Publications and Information Directorate; National Center for Biotechnology Information ahtibacterial, U. Instruments All melting point values are uncorrected and taken by open capillary method on a heating block Instrument. Medicinal plants are important sources of practical drugs for people throughout the year.

In vitro antibacterial activity of selected medicinal plants from lower Himalayas.

Effect of Adhatoda vasica massage in pyorrhoea. Table 4 Cytotoxic effect of Vasicine acetate against A lung adenocarcinoma cancer cell line. Avsica Uses of Medicinal Plants.

The antifungal agent Ketoconazole for fungi and antibacterial agent Streptomycin for bacteria were included in the assays as positive controls.

Zones of inhibition were recorded in millimetres and the experiment was repeated twice.